Title:

Tonsil - TONSIL-1 - CyCIF





Sample summary:

This is a small region of a human tonsil containing a germinal center and a section of crypt epithelium, obtained from the archives of the Department of Pathology, Brigham and Women’s Hospital as part of a discarded tissue protocol. It has been imaged with several immune cell markers as well as markers of general tissue structure.





Metadata template:

## Metadata about this sample  
### Demographics  
**Species:** Human  
**Vital Status:** [Alive/deceased]  
**Cause of death:** [if deceased]  
**Sex:** (Female, Male, Intersex)   
**Race:** [White, American Indian or Alaska Native, Black or African American, Asian, Native Hawaiian or Pacific Islander, Other]  
**Ethnicity:** [Hispanic or Latino, Not Hispanic or Latino]  

### Diagnosis  
**Age at Diagnosis:** [years]  
**Primary Diagnosis:**  
**Site of Resection or Biopsy:**  
**Tumor Grade:**  
**Stage (AJCC 8th Edition):**  

### Imaging  
**Imaging Assay Type:**  
**Fixative Type:**  
**Microscope:**  
**Objective:**  

## Publication and Data Availability  

### Attribution:  

**Please cite the publication and underlying data as:**  
Author, AA, Author, BB, Author CC, et al. *Article Title*. Journal. Year Volume(Issue): Page range, DOI:  [xx.xxx/yyyy](doi.org/myDOI) 

**Please cite this Minerva Story as:**    

*If the story has a DOI (via Dataverse or another repository)*: AA Author, BB Author, CC Author, etc. Story Title. Harvard Dataverse. Year, DOI: [xx.xxx/yyyy](doi.org/myDOI)  

*If the story does not have a DOI*: AA Author, BB Author, CC Author, etc. Story Title. Creation Year, URL.  





Site of Resection or Biopsy:

Tonsil





Microscope:

General Electric IN Cell Analyzer 6000





Objective:

Nikon 40X / 0.95NA





Attribution:

**Please cite the publication and underlying data as:**  
Du, Z., Lin, JR., Rashid, R. et al. Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging. Nat Protoc 14, 2900–2930 (2019). [doi:10.1038/s41596-019-0206-y](https://doi.org/10.1038/s41596-019-0206-y)





Channel descriptions:

DNA1: Nuclei
Keratin: Crypt epithelium
Ki-67: Proliferating immune cells
aSMA: Blood vessels
CD45: Immune cells (general)
CD20: B-cells
CD8A: Cytotoxic T-cells
CD4: T-helper cells
CD3D: T-cells (all)